I don't think anyone can give you an accurate answer for that. It really does depend on more factors than the number of active users. Their behavior will influence that too. Do they report lots of issues? Do they tend to add lots of attachments? Big ones? What else do you have on that machine?
Your best bet is to start with something like 60GB, and monitor how space increases over time. For instance, my 250-user instance grew by about 15GB in 18 months. Around 50,000 issues were created during that time.
You can keep it really low by turning attachments off, or even just limiting them to smaller sizes. The application installation is a moderately fixed size, the database doesn't really take up vast amounts of space, it's almost always the attachments that grow and chew up the space.
I have multiple projects that use variations of the same base workflow. The variations depend on the requirements of the project or issue type. The variations mostly come in the form of new statuses ...
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