Access is handled by the permission scheme which is applied to each project. If your users normally have access to all projects it means they are in a group that allows access. Remove them from the group and create a new group for them that only has access the that one project.
Permissions for Company Managed projects and Team Managed projects are handled differently.
For Company Managed projects Permission Schemes are used, and the primary permission for granting a user access to a project is the Browse Projects permission. To enable the group to see only one project you have to make sure that they are not assigned to any groups, roles, etc. that have the Browse Projects permission in the projects you don't want them to see.
If the one project you want them to see is also a Company managed project, then you need to ensure they do have the Browse Projects permission for that project. That may mean that one project needs a separate permission scheme.
Team Managed projects use a project-level Access setting.
You will need to check the Access setting for each Team Managed project and ensure that is set to a level that will result in these users not having access to each of those projects.
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