GOTTCHA2 app worked with bacterial database but not fungal database

[Brooke Linnehan]

Hi! I am using the GOTTCHA2 app for classifying taxonomy of metagenomic reads. I have 28 metatranscriptome files that I have entered into GOTTCHA and got successful results using the bacterial and viral database. However, when I go to run the same 28 files using the fungal database, I get the following error:

Server error: 'There is no taxonomy classification assignment against JGI_fungi.Refseq90_bact_cg.FUNGI.species.fna reference DB. You may try another reference DB.'

I would appreciate any help on how to get this to work with the fungal database. Thank you!

[Nic Brough -Adaptavist-]

Welcome to the Atlassian Community!

I've seen this a couple of other times, but I can't remember the actual organisation.  You're working with an app (and the bacterial and viral database) which is being run by an organisation that uses Atlassian software for Issue tracking and documentation.

I'm afraid you've clicked on "help with Jira by Atlassian" instead of "help with the database".  We in the community can talk a bit about using Jira, but not the database.  I suspect you probably need to raise in issue in that Jira, or talk to the org admins, but either way, we can't really help you.