I have done several tests with Subject lines.
My ticket Key is "SS-60", the title is "New Test for Email responses"
When acting as a customer and replying via email to updates on this ticket I have tested the following subject lines:
“SS-60” -> response was successfully added as comment to ticket
“RE: SS-60 New Test for Email responses” -> response failed to add as comment
"“SS-60 New Test for Email responses" -> response was successfully add as comment to ticket
"Re: SS-60” -> response failed to add as comment