Try navigating to the Program Room. In the center-top of the screen is a "View Configuration" button. Select that button and you'll be provided an option to toggle on the PI Checklist. Try that and see if this resolves your issue. ~ Derek
This will show you how to create a Checklist in Jira Align. To get a checklist to appear in a Program Room, make sure to select the following options during creation...
Shared with: Room
Portfolio: Select the applicable Portfolio (a Program Room checklist can be unique by Portfolio, meaning each Program in a Portfolio would use the same checklist).
Hope this helps!