In order to access my Jira Service Desk (cloud), does every end user create a JIRA account?

AB_Bank_Zambia

I have over 400 end users who are on our domain. Will all these user need to create a JIRA account in order to access my web portal? 

Jack Brickey

If these 400 are to be Customers then you simply need to add them as customers. It can be tedious w/ that many but if you can get all of their emails and paste into a CSV format you can easily copy and paste that into the "add customer" dialog window.