To check and compare a single value for a radio or dropdown custom field against input (ex. "No") using regular expression check validator use "^text$".
Result will allow transition to successfully happen if input on custom field matches the text entered within regex in quotations above.
For example, use ^One$ to block a transition for a radio custom field with options "One", "Two" and "Three" where either "Two" or "Three" being selected would throw an error to user but "No" would allow the transition.