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Simple solution is to disallow unassigned issues globally, but you can also make it mandatory in the field configurations on a project and issue basis.
If you want to do this during the workflow, like only making it mandatory when people close the issue, then you'll need to use a validator on the transition - that can do "if assignee empty, refuse to complete the move to closed". Usually though, people automate it - it's not uncommon to see "set assignee to reporter when closing the issue", so it's easy for the originator of the issue to find things later and you can report on who is currently responsible irrespective of status.