That doesn't really make any sense, but I guess you mean "when the trial period ends, what changes in my JIRA system?"
Basically, it stops working, you can't add or update your data or use most of the functions. If you buy and apply a licence, then you get all the functions back that you saw during the trial.
I have multiple projects that use variations of the same base workflow. The variations depend on the requirements of the project or issue type. The variations mostly come in the form of new statuses ...
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