Our domain controllers were upgraded to Windows Server 2008 R2 and now we are getting connection refused when trying to sync.
The Test Settings reports the configuration is fine.
Do you know if there is some configuration required on the Active Directory side?
No, there's no special configuration. However, since the AD upgrade process is about the LDAP SCHEME and security update procedures, I'd suggest reviewing all the LDAP struture and credential setting in JIRA settings.
For further assistances, I believe that you should raise a ticket at https://support.atlassian.com
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